Figure 5From: C-terminal in Sp1-like artificial zinc-finger proteins plays crucial roles in determining their DNA binding affinityRegular and competition EMSA assays for the binding of the purified 7ZFPs to the p16 promoter specific Probe-1. (a and b) The binding activity of 7ZFPs to the Probe-1 DNA was partially abolished after finger 3 (RSDHLSK) in the 7ZFPs Active-A and Active-B was replaced with finger-5 (QSGHLQR). Probe-2 is the fragment of p16 5′UTR region containing a postulated Sp1 binding site.Back to article page