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Figure 4 | BMC Biotechnology

Figure 4

From: C-terminal in Sp1-like artificial zinc-finger proteins plays crucial roles in determining their DNA binding affinity

Figure 4

Results of EMSA assay for the binding of the purified 7ZFPs to the p16 -specific Probe-1. (a) The binding activity of 7ZFPs to the Probe-1 DNA was abolished after the S to R mutation in finger 6 in the 7ZFPs Active-G and Active-I; Variant-(f6), variants with a S to R mutation of the key amino acid at the position +2 of the α-helix in finger-6 in 7ZFPs; (b) The binding activity of 7ZFPs to the Probe-1 DNA was abolished when their C-terminal 2ZF domain in was replaced with the middle 2ZF domain in the 7ZFP Active-B and Active-C, or the linker between finger-7 and the 6Histidine-tag in the C-terminal region was shorten from TGEKP to TG in the 7ZFP Active-K.

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