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Table 2 Data metrics from MiSeq sequencing of standard and modified Nextera library preparations

From: Improved workflows for high throughput library preparation using the transposome-based nextera system

  Genomes trial
Reference genome P. falciparum C. difficile E. coli M. tuberculosis
% GC 19 29 50 66
Size (Mb) 23.3 4.3 5.2 4.4
Sample Standard Reaction E Standard Reaction E Standard Reaction E Standard Reaction E
Yield (Mb) Q20 432 260 278 457 171 220 184 194
% Mapped Reads 96.6 96.8 97.8 98.4 96.5 96.6 95.6 96.0
% Duplicates 0.5 0.5 0.8 1.6 0.4 0.7 0.5 0.6
% GC 22.7 22 28.6 30.4 48.4 48.6 61.8 61.8
  1. Genome trial data metrics. Samples were pooled and sequenced on a 150 bp PE Miseq Run. Data from the standard and the modified reaction were compared and showed no significant differences between the library preparations.