Figure 4

Sequencing QC: genome coverage. Coverage across the genome for C. difficile (A,B), E. coli (C,D) and M. tuberculosis (E,F) libraries constructed with standard (A,C,E) and reaction E (B,D,F) Nextera preparation. The coverage is consistent between the two preparations, with both protocols producing good, even coverage across the genome. The large spikes seen in all three genomes probably represent repetitive regions and are consistent between the two preparations.