Figure 3

Library insert size. Libraries were constructed using the standard (A) or reaction E Nextera (B). Sequencing metrics showed the library insert sizes for C. difficile (i), E. coli (ii) and M. tuberculosis (iii) to be approximately 250-300 bp irrespective of the original TapeStation profile (Figure 2A and B). C. difficile produced a library with a shorter size distribution (~180 bp) in this experiment (Bi).