Step | Volume (mL)c | Solutiond |
---|---|---|
01 | 00 - 20 | De |
02 | 20 - 40 | Cf |
03 | 40 - 60 | A |
04 | 60 – 90 | E = Protein |
05 | 90 – 115 | A |
06 | 115 – 130 | Mix 5% B |
        →       120        Collect 2 mL | ||
07 | 130 - 180 | Gradient of 5 to 100% B |
08 | 180 - 200 | B |
        →        Divert to waste | ||
End of method, washing up of the machine, and log off |