Skip to main content
Figure 6 | BMC Biotechnology

Figure 6

From: Engineering of the LukS-PV and LukF-PV subunits of Staphylococcus aureusPanton-Valentine leukocidin for Diagnostic and Therapeutic Applications

Figure 6

Size Exclusion Chromatography (SEC) of 6His-LukS-PV. A AKTA Explorer Chromatographic plot during SEC purification of 6His-LukS-PV. NOTE: Vertical axis = absorbance, Horrizontal axis = Time (minutes). B Purity of 6His-LukS-PV following purification by SEC. Lane 1, 6His-LukS-PV completely pure without bands of impurity; Lane 2, water; Lane 3, Total soluble lysate of E. coli BL21(DE3)pLysS-pET-21d(+)-lukS-PV after induction with IPTG; Lane 4, E. coli BL21(DE3)pLysS-pET-21d(+)-lukS-PV before induction with IPTG, Lane M, Protein Mw standard.

Back to article page