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Figure 4 | BMC Biotechnology

Figure 4

From: Engineering of the LukS-PV and LukF-PV subunits of Staphylococcus aureusPanton-Valentine leukocidin for Diagnostic and Therapeutic Applications

Figure 4

BioLogic LP chromatogram during Ni++-affinity chromatographic purification of 6His-LukS-PV (upper) and 6His-LukF-PV (lower) in real-time. NOTE: AU = Absorbance unit, mS/cm = conductance of mobile phase. The inscriptions within the blue circles are the operational mode, i.e., S = start, H = hold, P = Pause, C = continue, and E = end. 6His-LukS-PV purification: The sign-post shows a low concentration of protein (0.8922 AU) in the eluate. B 6His-LukF-PV purification: The sign-post shows a high concentration of protein (5.000 AU) in the eluate.

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BMC Biotechnology

ISSN: 1472-6750

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