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Table 1 Purification of BGL-CBD from E. coli harboring pET-BGL-Linker-CBD

From: Enzymatic properties of Thermoanaerobacterium thermosaccharolyticum β-glucosidase fused to Clostridium cellulovoranscellulose binding domain and its application in hydrolysis of microcrystalline cellulose

Steps

Total protein (mg)

Total activity (U)

Specific activity (U/mg)

Yield (%)

Cell extract

158

3357

21.2

100

Heat treatment

89

2954

33.2

88%

Ni-NTA affinity

32

2578

80.5

77%

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