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Table 2 Phenolic compound contents in the cell wall fractions

From: Fibres from flax overproducing β-1,3-glucanase show increased accumulation of pectin and phenolics and thus higher antioxidant capacity

μg/g DW

Fraction

 

Vanillic acid

Vanillin

Coumaric acid

Syringic aldehyde

Ferulic acid

Vitexin

WSF

Nike

0.360 ± 0.048

0.108 ± 0.028

0.169 ± 0.065

0.152 ± 0.052

0.744 ± 0.142

0.757 ± 0.014

B14

0.260 ± 0.007

0.150 ± 0.045

0.103 ± 0.036

0.09 ± 0.013

0.811 ± 0.336

0.930 ± 0.322

CSF

Nike

0.071 ± 0.003

0.009 ± 0.001

0.038 ± 0.004

0.016 ± 0.003

0.162 ± 0.006

0.213 ± 0.014

B14

0.104 ± 0.032

0.016 ± 0.003*

0.055 ± 0.011

0.032 ± 0.018

0.295 ± 0.055*

0.326 ± 0.053*

NSF

Nike

0.258 ± 0.021

0.034 ± 0.009

0.150 ± 0.021

0.055 ± 0.009

0.635 ± 0.021

1.085 ± 0.085

B14

0.201 ± 0.014*

0.043 ± 0.008

0.12 ± 0.014

0.042 ± 0.003*

0.622 ± 0.012

1.022 ± 0.074

K1SF

Nike

1.771 ± 0.115

1.196 ± 0.240

0.431 ± 0.041

1.212 ± 0.071

2.306 ± 0.176

3.349 ± 0.270

B14

1.886 ± 0.530

1.917 ± 0.505

0.593 ± 0.032**

0.895 ± 0.11*

2.925 ± 0.281*

4.044 ± 0.361*

K4SF

Nike

0.477 ± 0.286

0.079 ± 0.01

0.046 ± 0.012

0.116 ± 0.048

0.145 ± 0.050

0.185 ± 0.047

B14

1.597 ± 0.199*

0.336 ± 0.034**

0.127 ± 0.009**

0.370 ± 0.045**

0.563 ± 0.073**

0.256 ± 0.034

  1. UPLC determination of the vanillic acid, coumaric acid, ferulic acid, vanillin, syringic aldehyde and vitexin contents in the cell wall fractions (WSF – water-soluble fraction, CSF – CDTA-soluble fraction, NSF – Na2CO3-soluble fraction, K1SF – 1 M KOH-soluble fraction and K4SF – 4 M KOH-soluble fraction) for fibres from transgenic flax line B14 and non-transgenic control flax Nike. Analyses were performed as described in the Methods section. Data represents the mean values ± SD from three independent experiments. The significance of the differences between the means was determined using Student’s t test (* - P < 0.05, ** - P < 0.01).
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BMC Biotechnology

ISSN: 1472-6750

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