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Figure 1 | BMC Biotechnology

Figure 1

From: A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones

Figure 1

Genomic location of fosmid WRM069dD11 insert. Upper panel: genes on the forward (purple exons) and reverse (green exons) strands of the insert from fosmid genomic clone WRM069dD11. Lower panel: An expanded region of the insert demonstrating the relative locations of the genes (boxed) F09E5.3, F09E5.15 (prdx-2) and EEED8.6 (ccpp-6) for which published, independent expression data was available. Locations of the genomic regions used to drive gfp expression in the transcriptional reporters described by McKay et al. (2003) ref [32] and Hunt-Newbury et al. (2007) ref [33] (light grey boxes) for F09E5.3, F09E5.15 and EEED8.6, strains BC14910, BC13145 and BC11803, respectively, and Isermann et al. (2004) ref [35] (dark grey box) for F09E5.15 are indicated (lower panel). Figure derived from WormBase.

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BMC Biotechnology

ISSN: 1472-6750

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