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Figure 3 | BMC Biotechnology

Figure 3

From: A vaccine grade of yeast Saccharomyces cerevisiae expressing mammalian myostatin

Figure 3

Analysis of MSTN expression in yeast strain JMY12 by western blotting. (A) A negative control from parental yeast strain JMY1 and a positive control of MSTN expression from Cu2+-inducible promoter were shown in lanes 1 and 2, respectively. Expression of MSTN from yeast strain JMY12 was shown in lane 3. In upper panel, MSTN expression was revealed by mouse polyclonal anti-MSTN antibody. In lower panel, western blotting for ß-actin was performed to ensure the equal loading of protein samples. (B) JMY12 stored for two years and recovered for the western blotting. In upper panel, MSTN expression was revealed by mouse polyclonal anti-MSTN antibody. In lower panel, western blotting for ß-actin was performed to ensure the equal loading of protein samples.

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