Construction of recombinant yeast strain JMY12. (A) JMY11: The success of chromosomal integration in two randomly selected yeast transformants was confirmed by PCR using primers Cyc3-F and NTS2-3’-R. (B) JMY12: Removal of KanMX4 expression cassette was confirmed by PCR using the same primers for identification of yeast strain JMY11. Four randomly selected yeast colonies were shown in lanes 2–4. A negative control was shown in lane 6. In both (A) and (B), DNA fragments were separated in 1% agarose gel and visualized by ethidium bromide staining. Lane 1: DNA molecular weight marker.