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Table 5 Overview of different Staphylokinase expression systems published to date

From: Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha

No

Expression system

SAK Productivity [mg/Lbroth]

Cultivation system

Volume [L]

SAK - glycosylation [Yes/No]

Reference

1

E. coli

> 50

(not defined)

2.7

No

[8]

2

E. coli

> 175

Fermenter

10-15

No

[19]

3a

B. subtilis

140

Shake flask

0.25

No

[20]

3b

B. subtilis

337

Fermenter

2

No

[20]

4a

P.pastoris

110

Fermenter

< 5

Partially yes

[13]

4b

E. coli

300-400

Shake flask

0.1

No

[13]

5

E. coli

2800

Fermenter

2

No

[21]

6

P.pastoris

~ 1000

Shake flask (w/o -tunicamycin)

0.05

Yes

[17]

7

P.pastoris

 

Shake flask (w - tunicamycin)

0.05

No

[17]

8

H. polymorpha

~ 1100

Fermenter

80

No

-