From: Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha
No | Expression system | SAK Productivity [mg/Lbroth] | Cultivation system | Volume [L] | SAK - glycosylation [Yes/No] | Reference |
---|---|---|---|---|---|---|
1 | E. coli | > 50 | (not defined) | 2.7 | No | [8] |
2 | E. coli | > 175 | Fermenter | 10-15 | No | [19] |
3a | B. subtilis | 140 | Shake flask | 0.25 | No | [20] |
3b | B. subtilis | 337 | Fermenter | 2 | No | [20] |
4a | P.pastoris | 110 | Fermenter | < 5 | Partially yes | [13] |
4b | E. coli | 300-400 | Shake flask | 0.1 | No | [13] |
5 | E. coli | 2800 | Fermenter | 2 | No | [21] |
6 | P.pastoris | ~ 1000 | Shake flask (w/o -tunicamycin) | 0.05 | Yes | [17] |
7 | P.pastoris | Shake flask (w - tunicamycin) | 0.05 | No | [17] | |
8 | H. polymorpha | ~ 1100 | Fermenter | 80 | No | - |