Table 1 Overview of applied cultivation systems and their usage described herein
From: Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha
Cultivation scale [L] | Cultivation dimension | Cultivation system | Optimization parameter | Max. rSAK-2 conc. [mg/L] | Cell density [OD600] | Time [h] | Specific productivity [mg/OD600/h] | Media |
|---|---|---|---|---|---|---|---|---|
0.001 | Microtiter plate | Biolector | pH – media – feeding strategy | 90 | n.d. | n.d. | n.d. | SYN6 + wheat peptone |
0.1 | Shake flask (batch) | - | Media | 31 | 35 | 72 | 0.012 | SYN6.4 |
Shake flask (fed-batch) | - | Feed composition - media | 200 | 130 | 48 | 0.032 | SYN6.46d | |
0.300 | Parallel fermenter system | DASGIP | pH – media –feeding strategy/composition | 423 | 291 | 48 | 0.030 | SYN6.46d |
2 | Fermenter | Sartorius Biostat B Plus 3L | pH – feed rate/composition – fermentation duration | 1212 | 263 | 75 | 0.061 | SYN6.46d |
8 | Fermenter | Sartorius Biostat C 15L | Upscale | 1081 | 340 | 70 | 0.045 | SYN6.46d |
80 | Fermenter | Sartorius Biostat C 150L | Upscale | 1109 | 370 | 70 | 0.043 | SYN6.46d |