Skip to main content

Table 1 Overview of applied cultivation systems and their usage described herein

From: Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha

Cultivation scale [L] Cultivation dimension Cultivation system Optimization parameter Max. rSAK-2 conc. [mg/L] Cell density [OD600] Time [h] Specific productivity [mg/OD600/h] Media
0.001 Microtiter plate Biolector pH – media – feeding strategy 90 n.d. n.d. n.d. SYN6 + wheat peptone
0.1 Shake flask (batch) - Media 31 35 72 0.012 SYN6.4
Shake flask (fed-batch) - Feed composition - media 200 130 48 0.032 SYN6.46d
0.300 Parallel fermenter system DASGIP pH – media –feeding strategy/composition 423 291 48 0.030 SYN6.46d
2 Fermenter Sartorius Biostat B Plus 3L pH – feed rate/composition – fermentation duration 1212 263 75 0.061 SYN6.46d
8 Fermenter Sartorius Biostat C 15L Upscale 1081 340 70 0.045 SYN6.46d
80 Fermenter Sartorius Biostat C 150L Upscale 1109 370 70 0.043 SYN6.46d