Figure 5

Biomass growth over time of a glycerol fed-batch culture and a methanol induced culture, respectively, grown in SYN6 media. The Fed-Batch culture was pulsed twice at t = 1 and 2 with a mixture of 10 g/L glycerol and 10 g/L peptone (final concentration). At four points of time (1–4) a sample was taken. For the other culture, methanol was given once (1) at a final concentration of 10 g/L. Samples were taken at t = 1 and 3. Fermentation was carried out at 1mL scale in the BioLector® system. Samples were analysed in a Western Blot for rSAK-2 expression (5 μL supernatant loaded). Standards (std) are drawn for the individual gels. Final lanes were loaded with THR-174 (~109 ng).