Figure 4From: Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorphaWestern Blot analysis of rSAK-2, expressed in SYN6 medium supplemented with different plant peptones and a comparative fermentation without peptone (5 μL supernatant loaded). Sampled: a) at the end of exponential phase, b) after 2 hours and c) after 5 hours after the entry in stationary phase. Highest results from a 1 mL micro scale batch fermentation in the BioLector® system were attained with wheat peptone (17) followed by vegetable peptone (1). Final lanes were loaded with THR-174 (~109 ng).Back to article page