Figure 2

IMAC of SDS-denatured proteins is a generic method for protein purification. Coomassie stained SDS-PAGE gels that show the purity of seventeen purified proteins. Lanes are labelled as follows: BSA 0.1 and BSA 1: Samples were prepared from BSA stock solutions containing 0.1 mg/ml and 1 mg/ml BSA respectively by adding 40 μl 5X SDS Sample buffer to 40 μl of stock solution. 15 μl each were loaded onto the gel - containing 0.75 and 7.5 μg BSA total. Numbers 165–184 indicate the expression construct and protein purified. See Table 1 for details of expression constructs. Samples were prepared and loaded as for the BSA control samples.