Figure 3

Flow cytometry demonstrated the immunoreactivity of the TfRscFv-GAL4 to TfR expressed on the surface of different tumor cells. The cells were incubated with either negative control mouse non-specific IgG, positive control Mouse anti-TfR monoclonal antibody, TfRscFv-GAL4 fusion protein or mouse anti-GAL4 antibody used for detection of GAL4 domain, followed by FITC-labeled goat anti- mouse IgG. The proportion of FITC positive cell to the whole cell was calculated as showed in A, The statistical results presents there was significantly difference in the binding rate between the TfRscFv-GAL4 group and the negative control groups as showed in B (p<0.01), these results are representatives of four studies that have been done.