BMC Biotechnology

Table 1 Reaction times and yields for the oligosaccharide synthesis by the fructosyltransferase Suc1 from A. niger

From: Chemo-enzymatic synthesis and in vitro cytokine profiling of tailor-made oligofructosides

	t	Conversion
	[min]	[% mol mol^-1]
1-kestose	18	81
1-nystose	60	93
MF₂	60	71
MF₃	180	87
GF₂	420	44
GF₃	960	65
XF₂	20	75
XF₃	120	94
FF₂	60	65
FF₃	120	88

Reaction conditions were Suc1 enzyme dilution 1:50 (v/v), 500 mM sucrose analogue to be converted in Sörensen`s phosphate buffer (50 mM, pH 5.6) at 45°C and 200 rpm. The reaction time depending on the oligosaccharide to be synthesized is indicated.

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ISSN: 1472-6750

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