Figure 3
From: Chemo-enzymatic synthesis and in vitro cytokine profiling of tailor-made oligofructosides
Synthesis of the oligofructosides capped by fucose. A, the acceptor reaction for the fucosyl-containing disaccharide Fuc-Fru was analyzed by HPAEC. Reaction conditions were: Fucose (1.2 M), sucrose (600 mM) in phosphate buffer after Sörensen (50 mM, pH 6.6), 10 mg l-1 fructosyltransferase SacB, at 200 rpm and 37°C for 2 h. B, the same acceptor reaction as in A shown as HPAEC chromatogram. Carbohydrates corresponding to the peaks are indicated. Analysis time points are indicated as follows. Red, 0 min, blue, 10min, green, 30 min and black, 2 h reaction time. C, The transfructosylation reaction yielding the fucosyl-capped tri- and tetrasaccharides Fuc-(Fru)2 and Fuc-(Fru)3 was performed using Suc1-containing culture supernatant 1:50 (v/v), 500 mM Fuc-Fru in Sörensen`s phosphate buffer (50 mM, pH 5.6), at 45°C and 200 rpm.