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Table 2 PCR analysis of BC1 and S1 plants

From: The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis

TR1-Parent line Plants tested Positive for target locus a Positive for excision b Positive for excision and negative for recombinase gene c Positive for recombinase gene and negative for target locus d
Bxb1.9.7 48 36 20 0 1
Bxb1.9.8 18 14 14 5 0
Bxb1.9.11 6 6 6 0 0
Bxb1.9.17 40 32 32 15 1
Bxb1.9.23 51 41 36 0 9
Bxb1.9.26 49 46 22 0 0
Bxb1.9.29 18 16 12 0 1
Bxb1.9.31 44 10 9 0 24
Bxb1.9.44 32 28 26 17 0
Bxb1.12.52 27 14 8 0 6
Bxb1.12.61 9 6 6 1 2
Bxb1.12.69 8 2 2 0 3
Bxb1.12.72 9 4 1 0 3
Bxb1.12.73 9 3 3 1 6
Bxb1.12.74 9 9 4 0 0
Bxb1.12.79 9 8 6 1 0
Bxb1.12.81 9 7 3 0 0
Bxb1.12.82 9 8 7 0 1
Bxb1.12.83 9 9 3 1 0
Bxb1.28.1 27 26 22 6 0
Bxb1.28.2 18 18 14 0 0
Bxb1.28.3 16 16 11 1 0
Bxb1.28.4 9 8 8 3 0
Bxb1.28.6 26 24 24 11 0
Bxb1.28.7 17 15 13 0 0
Bxb1.28.8 3 3 3 2 0
Bxb1.28.9 18 17 17 4 0
Bxb1.28.10 23 22 16 3 0
Bxb1.28.11 8 7 7 2 0
Bxb1.35.1 18 9 5 0 0
Bxb1.35.2 36 28 17 7 0
Bxb1.35.3 103 47 27 1 9
Bxb1.35.15 23 19 14 5 0
Bxb1.35.29 24 23 23 8 0
Bxb1.35.32 23 23 23 0 0
Bxb1.35.33 23 19 16 1 0
Bxb1.35.36 24 23 23 5 0
Bxb1.35.41 15 14 9 0 0
Bxb1.35.44 17 17 16 0 0
  1. a Primers eand fyielded the 1.23 and/or 0.43 kb target fragment
  2. b Primers eand fyielded the 0.43 kb excision fragment
  3. c Primers gand hfailed to detect the 0.72 kb Bxb1 fragment
  4. d Primers gand hyielded the 0.72 kb Bxb1 fragment and primers eand ffailed to detect the 1.23 and/or 0.43 kb target fragment