PCR analysis for detection of site-specific recombination and the recombinase transgene in the retransformation (TR
) and back cross (BC
) generations. PCR reactions (a, c) with primers eand f(Figure 1) or (b, d) with primers gand h(Figure 1) on representative plant DNAs. a, b) retransformed TR1-Bxb1.9.17 lines. c, d) Back crossed line BC1-Bxb126.96.36.199. Control lanes are B (blank, no DNA); E (excision, pN6-Bxb1exc DNA); N (no excision, pN6-Bxb1); P (pCOXS3-Bxb1). c) Lane numbers underlined indicate presence of excision event in the absence for the Bxb1 gene.