Scalable production of biliverdin IXα by Escherichia coli

BMC Biotechnology

Table 1 Biliverdin production by E. coli (HO1) and E. coli (mHO-1) growing in LB medium supplemented with various carbon sources

*E.coli* Strain	Carbon Source	Conc. %	IPTG^a	Pigment^b
BL21(HO1)	D-glucose	1	+	pale green
		2	+	pale green
		5	+	yellow green
	sucrose	1	+	brown
	D-mannitol	1	+	pale green
		2	+	pale green
		5	+	green
	D-sorbitol	1	+	yellow green
		5	+	yellow green
		10	+	yellow green
	lactose	1	-	pale green
		2.5	-	green
		5	-	yellow green
		10	-	yellow green
	D-mannitol, D-glucose	1,1	+	pale green
	sucrose, D-glucose	1,1	+	pale green
	D-mannitol, D-sorbitol	2.5, 2.5	+	green
	D-mannitol, D-sorbitol	2, 5	+	green
	D-mannitol, D-sorbitol	5, 5	+	green
	lactose, D-glucose	2, 2	-	green
	succinate	5	+	pale green
	malate	5	+	pale green
	citrate	5	+	pale green
	L- glutamate	5	+	pale green
	L- glutamate	5	+	yellow green
BL21(mHO1)	D-glucose	1	+	green
	D-mannitol	5	+	green
	lactose	2.5	-	green
	lactose, D-glucose	2, 2	-	green

^aIsopropyl-β-thiogalactoside (IPTG), 0.5 mM added (+), or not added (−).
^b Biliverdin concentrations were 2 to 4mg L^-1 (green), <2 to >0.2 mg L^-1 (pale green) and <0.2mg L^-1 (yellow green and brown).

ISSN: 1472-6750