Skip to main content

Table 6 Comparative GUS activity (pmolMU/min/μg protein) observed with 35S and nos promoter carrying constructs with different 5'UTRs in transformed cotton callus

From: A 28 nt long synthetic 5UTR (synJ) as an enhancer of transgene expression in dicotyledonous plants

Construct

Expt No.

No. of callus tested

Gus activity (pmolMU/min/μg protein)

Range

Mean ± S.E.

35S(nos)

I

27

67.5 - 1046.8

465.6 ± 47.1

II

30

48.8 - 691.7

274.8 ± 30.8

III

22

101.3 - 1077.0

580.6 ± 60.8

nos(nos)

I

20

22.0 - 100.1

54.4 ± 4.9

II

22

35.5 - 98.4

64.6 ± 3.4

III

22

28.8 – 114.8

70.4 ± 6.4

35S(synJ)

I

21

304.5 - 1916.9

989.8 ± 93.3

II

29

222.5 - 2216.3

869.5 ± 99.1

III

20

60.5 - 2389.0

1144.9 ± 254.0

nos(synJ)

I

16

37.9 - 314.6

131.2 ± 16.2

II

22

54.6 - 364.7

177.2 ± 17.1

III

27

73.7 - 577.5

287.7 ± 23.1

35S(RT)

I

25

2.7 - 12.7

7.8 ± 0.54

II

26

3.3 – 11.3

7.03 ± 0.42

nos(RT)

I

23

4.9 – 15.6

9.33 ± 0.6

II

25

5.9 – 19.4

9.4 ± 0.57