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Table 5 Examples of GUS activity driven by 35S promoter observed in different tissues of tobacco plants as reported in different studies

From: A 28 nt long synthetic 5UTR (synJ) as an enhancer of transgene expression in dicotyledonous plants

Reference

Gus expression studied in

Range of gus activity (pmol MU/min/μg protein)

Sawant et al. (2001)*

Tobacco leaves

~0.13

De Amicis et al. (2007)*

Tobacco leaves

0.02-0.5

Outchkourov et al. (2003)*

Tobacco leaves

~31.7

Benfey et al. (1989)*

Tobacco transgenics (seedlings)

39.6-63.8

Jefferson et al. (1987)*

Tobacco leaves, stem & roots

0.2-0.6

Xu et al. (2010)*

Tobacco leaves

2.0-4.0

Sanger (1990)*

Tobacco leaves

~9.03

Present study

Tobacco leaves

2.56-3257.0

Tobacco stems

16.0-1547.0

Tobacco roots

23.3-14955.0

  1. *In these papers GUS activity is expressed in different units. The value has been converted to pmolMU/min/µg protein for comparing it with the results of the present study.