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Table 4 Comparative GUS activity recorded in different tissues of transgenic tobacco lines transformed with constructs 35S(synJ), 35S(MsynJ) and 35S(synM)

From: A 28 nt long synthetic 5UTR (synJ) as an enhancer of transgene expression in dicotyledonous plants

Tissue analyzed

GUS activity (pmolMU/min/μg protein)

35S(synJ)

35S(MsynJ)

35S(synM)

Leaf

Range

15.9 - 3999.8

48.5 - 888.1

15.7 - 805.2

No. of lines tested

21

18

22

% Lines showing GUS activity >1000

33.3

0

0

Mean ± S.E

951.3 ± 198.8

363.4 ± 54.6

329.9 ± 51.4

Stem

Range

3.3 - 2380.0

4.6 - 739.4

2.9 - 482.5

No. of lines tested

21

20

25

% Lines showing GUS activity >500

61.9

25.0

0

Mean ± S.E

673.3 ± 136.8

321.3 ± 49.9

140.1 ± 27.5

Root

Range

4.4 - 7297.0

3.3 – 975.0

6.2 - 734.2

No. of lines tested

21

19

24

% Lines showing GUS activity >1000

81.0

0

0

Mean ± S.E

1914.9 ± 371.9

424.4 ± 75.3

297.4 ± 45.9