Figure 2
From: A 28 nt long synthetic 5′UTR (synJ) as an enhancer of transgene expression in dicotyledonous plants
GUS activity observed in cotton callus transformed with (A) 35S(RT), (B) 35S(pBI) and (C) 35S(synJ). GUS activity was studied after 40–45 days of transformation. GUS activity recorded in callus transformed with 35S(synJ) was many fold higher than 35S(RT) and 35S(pBI) (see scales of Y-axis). Each circle represents the absolute GUS activity (pmolMU/min/μg protein) measured in one independent callus. GUS activity in this as well as in all the following figures is a mean of two independent reactions carried out for two time points. N in this figure and in later figures denotes the number of callus analyzed in each case.