Figure 1
From: A 28 nt long synthetic 5′UTR (synJ) as an enhancer of transgene expression in dicotyledonous plants
Schematic representation of binary vectors (within T-DNA borders) developed for the transformation of tobacco and cotton. The left and right borders of the T-DNA are designated as LB and RB, respectively. The selection marker gene (nptII) is driven by the nos promoter (Pnos) which also has a polyA signal of octopine synthase gene (ocspA). Different promoters (35S and nos) and reporter genes (gus and gfp) were used for creating various expression cassettes with a polyA signal of 35S (35SpA). A given combination of promoter and 5′UTR constituted an upstream regulatory module (URM).