Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig

BMC Biotechnology

Table 5 Nucleofection results PKC2109, PKCm, PFF26 and PEF0110

Cell Number	DNA amount [μg]	Program	Quality	Cells in suspension	Fluor. intensity	Transfection efficiency [%]
PKC2109
0.5x10⁶	2	A24	2	1	+	28
	2	U23	2	2	+	60
	2	U12	1	1	++	63
	2	T16	2	1	+	54
	2	V13	1	1	++	59
0.5x10⁶	1	U12	1	1	+	36
	5	U12	1	1	++	70
	10	U12	1	1	+++	77
	20	U12	2	1	++	89
1.0x10⁶	5	U12	1	1	++	68
	10	U12	1	1	+++	88
	20	U12	2	2	+++	83
PKCm
0.5x10⁶	2	U12	1	1	++	49
	2	T16	1	1	++	50
	2	V13	2	1	+++	66
PFF26
0.5x10⁶	2	U12	1	1	+	53
	2	T16	1	1	+	50
	2	V13	2	2	++	77
PEF0110
0.5x10⁶	2	U12	1	1	+	15
	2	T16	1	1	+	11
	2	V13	2	2	+	34

Scale for the assessment of cell quality: 1- excellent-good; 2- cells are bit stretched/strongly fibroblast-like; 3- stretched cells with vacuoles; 4- cells with very spindle-shaped morphology 5- extreme spindle-shaped with vacuoles; Scale for the assessment of cells in suspension: 0- none; 1- low number of cells; 2- a lot of cells; Fluor. = Fluorescence.

ISSN: 1472-6750