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Table 4 Electroporation results PKC2109

From: Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig

Cell number DNA amount [μg] Quality Cells in suspension Fluor. intensity Transfection efficiency [%]
0.5x106 1 3 1 + 28
5 3 2 + 46
10 3 2 ++ 47
1.0x106 1 4 2 + 24
5 4 2 + 22
10 4 2 ++ 30
20 5 2 +++ 54
  1. Scale for the assessment of cell quality: 1- excellent to good; 2- cells are bit stretched/strongly fibroblast-like; 3- stretched cells with vacuoles; 4- very spindle-shaped morphology 5- extreme spindle-shaped cells with vacuoles; Scale for the assessment of cells in suspension: 0- none; 1- low number of cells; 2- a lot of cells; Fluor. = Fluorescence. Endotoxin-free purified DNA was used; Voltage: 230V.