From: Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig
DNA amount [μg] | Plus reagent [μl] | Ratio lipid to DNA | Quality | Cells in suspension | Fluor. intensity | Transfection efficiency [%] |
---|---|---|---|---|---|---|
0.25 | 0.25 | 1.0:1 | 1 | 0 | + | 0.6 |
2.0:1 | 2 | 0 | + | 0.5 | ||
2.5:1 | 2 | 0 | + | 3.1 | ||
3.0:1 | 2 | 0 | + | 3.8 | ||
4.0:1 | 4 | 0 | ++ | 26 | ||
0.5 | 0.5 | 1.0:1 | 2 | 0 | + | 2.6 |
2.0:1 | 4 | 1 | + | 26 | ||
2.5:1 | 3 | 1 | ++ | 50 | ||
3.0:1 | 5 | 2 | ++ | 52 | ||
0.5 | 1.0 | 2.5:1 | 4 | 1 | ++ | 24 |
3.0:1 | 4 | 2 | ++ | 45 | ||
0.75 | 0.75 | 1.0:1 | 4 | 0 | ++ | 28 |
2.0:1 | 5 | 1 | ++ | 37 | ||
2.5:1 | 5 | 1 | ++ | 28 | ||
3.0:1 | 5 | 1 | ++ | 21 |