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Table 2 Lipofection results PKC2109

From: Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig

DNA amount [μg]

Plus reagent [μl]

Ratio lipid to DNA

Quality

Cells in suspension

Fluor. intensity

Transfection efficiency [%]

0.25

0.25

1.0:1

1

0

+

0.6

2.0:1

2

0

+

0.5

2.5:1

2

0

+

3.1

3.0:1

2

0

+

3.8

4.0:1

4

0

++

26

0.5

0.5

1.0:1

2

0

+

2.6

2.0:1

4

1

+

26

2.5:1

3

1

++

50

3.0:1

5

2

++

52

0.5

1.0

2.5:1

4

1

++

24

3.0:1

4

2

++

45

0.75

0.75

1.0:1

4

0

++

28

2.0:1

5

1

++

37

2.5:1

5

1

++

28

3.0:1

5

1

++

21

  1. Scale for the assessment of cell quality: 1- excellent to good; 2- no vacuoles and lipids detectable, cells are bit stretched; 3- partially vacuoles and lipids, stretched cells; 4- many vacuoles and lipids, stressed cells, partially enlarged cells; 5- a lot of large vacuoles and lipids, altered morphology and enlarged cells; Scale for the assessment of cells in suspension: 0- none; 1- low number of cells; 2- a lot of cells; Fluor. = Fluorescence.