Potential of primary kidney cells for somatic cell nuclear transfer mediated transgenesis in pig

BMC Biotechnology

Table 1 In vitro development competence of cloned embryos using PKCm, PEF0110 and PFF26

Donor cells	Fusion Rate (%)	Reconstructed embryos cultured	Reconstructed embryos developed to blastocyst stage (%)				Cell number of blastocysts [mean+/−SEM]
Donor cells	Fusion Rate (%)	Reconstructed embryos cultured	hatched	not hatched	degen.	overall	Cell number of blastocysts [mean+/−SEM]
PKCm	85/90	81	10	6	1	17 (21.0)^a	43.5±4.2
PKCm	(94.4)^a	81	10	6	1	17 (21.0)^a	43.5±4.2
PEF0110	70/85	70	2	1	0	3 (4.3)^b	23.5±7.5
PEF0110	(82.4)^b	70	2	1	0	3 (4.3)^b	23.5±7.5
PFF26	74/86	77	5	2	0	7 (9.1)^a,b	56.1±9.5
PFF26	(86.0)^a,b	77	5	2	0	7 (9.1)^a,b	56.1±9.5

Analysis of blastocysts on day 7. Values with different superscript were significantly different (P<0.05), SEM = standard deviation. degen.=degenerated.

ISSN: 1472-6750