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Figure 7 | BMC Biotechnology

Figure 7

From: Enhanced recombinant protein production and differential expression of molecular chaperones in sf-caspase-1-repressed stable cells after baculovirus infection

Figure 7

RT-PCR analysis of molecular chaperones in baculovirus-infected Sf -caspase-1-repressed stable cells. Total mRNA of baculovirus-infected cells, Sf9 and Sf9/pIBdsCasp-1, at each hour post infection (hpi) were extracted and analyzed by RT-PCR. The marked numbers below images were relative mRNA level (%), which were normalized with rRNA and compared with corresponding mRNA level of Sf9 cell at 0 hpi. The corresponding specific primer sequences and PCR cycles were presented in Table 1. dsC1, Sf9/pIBdsCasp-1 cells; act, β-actin.

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