Skip to main content

Advertisement

Table 1 Protein identification table

From: NanoUPLC-MSE proteomic data assessment of soybean seeds using the Uniprot database

Uniprot code Description Protein MW ScoreAVG ProductsAVG PeptidesAVG Fmol Covariance NgramAVG Repeate Rate % of TSP
P04776 Glycinin G1 OS Glycine max GN GY1 PE 1 S 56333.71 147104.4 523.25 38.5 0.02 85.28 4 17.68
Q549Z4 Proglycinin A2B1 OS Glycine max PE 2 SV 54961.11 69391.44 427.25 38.25 0.67 55.32 4 11.47
Q4LER5 Beta conglycinin alpha subunit Fragment 70348.96 105347.27 301.75 54.25 0.75 32.68 4 6.78
Q3V5S6 Beta conglycinin alpha subunit OS Glycin 70569.34 104417.56 286.5 53.75 0.72 30.91 4 6.41
P04405 Glycinin G2 OS Glycine max GN Gy2 PE 1 S 54961.11 63666.28 370 36.5 1.97 19.12 4 3.96
Q7XXT2 Prepro beta conglycinin alpha prime subu 72532.23 28368.36 295 66 0.67 18.17 4 3.77
P04347 Glycinin OS Glycine max PE 1 SV 1 58412.39 23431.64 191.5 53 0.22 15.99 4 3.31
P02858 Glycinin G4 OS Glycine max GN GY4 PE 1 S 64043.42 31521.33 290.75 60 0.26 14.25 4 2.95
Q9SB12 Glycinin OS Glycine max PE 3 SV 1 58685.70 29853.17 251 68.75 0.48 13.30 4 2.76
Q9S9D0 Glycinin G4 subunit OS Glycine max PE 3 64135.62 32357.33 309 60.25 0.31 11.44 4 2.37
Q04672 Sucrose binding protein OS Glycine max G 60921.87 11679.68 186.25 33.5 0.02 11.37 4 2.36
Q9ATY1 Kunitz trypsin inhibitor OS Glycine max 24434.59 35543.40 112.5 13.75 0.17 11.34 4 2.35
Q948Y0 Beta conglycinin alpha prime subunit OS 72423.19 17151.17 223.5 65.75 0.75 10.95 4 2.27
Q948X9 Beta conglycinin alpha subunit OS Glycin 72760.60 15765.20 186.25 44.25 0.75 9.25 4 1.92
P05046 Lectin OS Glycine max GN LE1 PE 1 SV 1 30928.02 10795.06 63.75 10.25 0.01 7.95 4 1.65
Q94LX2 Beta conglycinin alpha subunit OS Glycin 70591.40 113386.25 365.5 51.75 1.01 7.57 4 1.57
P00489 GLYCOGEN PHOSPHORYLASE MUSCLE FORM EC 97671.64 3801.66 161.5 37.5 0.00 7.33 4 1.52
P11827 Beta conglycinin alpha chain OS Glycine 74610.54 11508.88 185.25 65 0.86 7.31 4 1.52
C6SWW4 Putative uncharacterized protein OS Glyc 22731.98 17601.57 100.5 12.25 0.07 7.12 4 1.48
P25974 Beta conglycinin beta chain OS Glycine m 50609.06 19512.84 186.25 34 0.71 7.10 4 1.47
Q39898 Kunitz trypsin inhibitor OS Glycine max 24361.54 54750.93 144.25 14.5 1.16 6.72 4 1.39
C6T9L1 Putative uncharacterized protein OS Glyc 50621.07 18189.66 182.5 33.25 0.96 6.30 4 1.31
P93708 Glycinin OS Glycine max GN Gly A3B4 PE 2 58691.62 39274.08 237.25 62.5 1.18 5.91 4 1.23
P13916 Beta conglycinin alpha chain OS Glycine 70578.31 113406.84 362 49.5 0.76 5.75 4 1.19
B3TDK4 Lipoxygenase OS Glycine max PE 3 SV 1 94639.64 4359.21 144.25 35.75 0.03 5.63 4 1.17
Q93VL9 Beta conglycinin beta subunit OS Glycine 50580.97 17965.49 159.75 32 0.72 5.29 4 1.10
Q852U5 Glycinin A1bB2 445 OS Glycine max PE 2 S 54845.14 19226.30 97 16 0.33 4.59 4 0.95
P13917 Basic 7S globulin OS Glycine max GN BG P 47134.49 4532.62 62.5 14 0.03 3.51 4 0.73
P01070 Trypsin inhibitor A OS Glycine max GN KT 24290.46 40525.39 125 14 1.97 3.36 4 0.70
Q70EM0 Dehydrin OS Glycine max GN lea D 11 PE 3 23787.84 11066.56 101.5 19 0.02 3.24 4 0.67
Q0MUU5 Beta conglycinin alpha subunit OS Glycin 70172.28 24742.62 233.5 63.25 2.00 3.14 4 0.65
C0KG62 Mutant glycinin A3B4 OS Glycine max PE 2 60494.82 42247.79 242.5 62.25 2.00 2.46 4 0.51
Q7GC77 Glycinin A3B4 subunit OS Glycine max PE 58643.62 50693.51 331 57.5 2.00 2.39 4 0.49
Q53WV6 Napin type 2S albumin 3 OS Glycine max P 19030.31 21340.11 82.75 13 1.17 2.19 4 0.45
Q43452 Glycinin OS Glycine max GN Gy4 PE 3 SV 1 64389.76 32891.71 289.25 50.5 2.00 2.19 4 0.45
Q852U4 Glycinin A1bB2 784 OS Glycine max PE 2 S 54868.22 18987.27 81.75 13.5 1.30 1.90 4 0.39
P10538 Beta amylase OS Glycine max GN BMY1 PE 1 56485.05 1358.99 57 23.5 0.72 1.87 4 0.39
Q8RVH5 Basic 7S globulin 2 OS Glycine max PE 1 47889.37 2086.68 37.25 11.75 0.38 1.87 4 0.39
Q39853 Soybean beta conglycinin alpha subunit F 24453.59 88244.12 88.5 9.25 1.89 1.77 4 0.37
C6SYA7 Putative uncharacterized protein OS Glyc 19027.27 23176.74 87 15 1.19 1.75 4 0.36
Q39871 Late embryongenesis abundant protein OS 50643.91 919.20 40.5 14 0.17 1.65 2 0.34
C6T7B0 Putative uncharacterized protein Fragmen 48939.81 23743.45 190.25 54.25 1.16 1.61 4 0.33
C0J370 Ribulose bisphosphate carboxylase large 52835.80 2789.04 34.25 9.25 0.75 1.60 4 0.33
C6TKH0 Putative uncharacterized protein OS Glyc 32117.31 1246.92 38.75 10 0.03 1.54 4 0.32
Q9SB11 Glycinin OS Glycine max GN A5A4B3 PE 2 S 64253.61 38444.74 381.5 49 1.98 1.24 4 0.26
Q39858 Soybean glycinin A3 B4 subunit Fragment 27467.17 26110.47 58.5 10.25 2.00 1.20 4 0.25
Q39816 7S storage protein alpha subunit OS Glyc 27538.85 8464.43 117.75 29 1.19 0.97 4 0.20
Q39805 Dehydrin like protein OS Glycine max PE 23717.75 10257.65 91.5 17 1.61 0.96 4 0.20
P19594 2S albumin OS Glycine max PE 1 SV 2 19030.31 20001.72 82 14.75 1.74 0.93 4 0.19
P11828 Glycinin G3 OS Glycine max GN GY3 PE 3 S 54869.09 20193.73 96 16.5 0.83 0.86 4 0.18
C6TDF5 Putative uncharacterized protein OS Glyc 42166.14 2011.92 45 12.5 0.61 0.81 2 0.17
Q39876 Maturation associated protein OS Glycine 23713.76 11376.82 107.75 19.25 1.99 0.80 4 0.17
Q42795 Beta amylase OS Glycine max PE 1 SV 1 56413.93 1363.11 54.75 22.5 1.99 0.73 4 0.15
Q42447 Maturation protein OS Glycine max GN MAT 25658.99 3286.16 26.25 6.25 0.73 0.64 4 0.13
Q9SEK9 Seed maturation protein PM25 OS Glycine 25842.80 1323.54 30.33 7.66 0.37 0.63 3 0.13
Q9SP11 Sucrose binding protein homolog S 64 OS 56176.59 2088.61 62.5 24 0.69 0.62 4 0.13
Q9XET0 Putative uncharacterized protein OS Glyc 15154.43 2553.76 27.75 5.75 0.03 0.57 4 0.12
Q9ATY0 Truncated Kunitz trypsin inhibitor OS Gl 16134.39 18524.95 49.5 7.25 2.00 0.57 4 0.12
C6T1Q7 Putative uncharacterized protein OS Glyc 18394.94 4148.8 41.25 10.75 0.67 0.55 4 0.11
Q9LLQ6 Seed maturation protein PM34 OS Glycine 32052.25 1013.75 24.5 10 0.39 0.55 2 0.11
P01064 Bowman Birk type proteinase inhibitor D 10323.16 4799.89 22.25 4.5 0.68 0.54 4 0.11
P25273 Kunitz type trypsin inhibitor KTI2 OS Gl 23085.45 2073.41 19.75 7.25 0.87 0.51 4 0.11
C6T588 Putative uncharacterized protein OS Glyc 16817.85 2001.68 23 6.25 0.14 0.49 4 0.10
A1KR24 Dehydrin OS Glycine max GN LEA 2 D11 PE 25384.72 3672.93 34.25 8.75 0.44 0.46 4 0.10
P09439 Seed lipoxygenase 2 OS Glycine max GN LO 97430.94 763.76 45 27 1.41 0.40 2 0.08
C6TB67 Putative uncharacterized protein OS Glyc 23271.56 1060.30 16 5.33 0.03 0.40 3 0.08
P27066 Ribulose bisphosphate carboxylase large 53066.07 2905.08 31.75 8.75 2.00 0.37 4 0.08
P01063 Bowman Birk type proteinase inhibitor C 9999.68 5359.28 33.25 4.25 0.10 0.34 4 0.07
B3TDK5 Lipoxygenase OS Glycine max PE 3 SV 1 97068.50 753.71 44 26.5 1.41 0.33 2 0.07
Q39875 Soybean lipoxygenase 1 Fragment OS Glyci 36808.29 1111.12 23 12.333333 1.65 0.31 3 0.06
Q9SBA9 Bowman Birk proteinase inhibitor Fragmen 5100.41 8774.56 15 1 0.03 0.31 4 0.06
Q76B18 Kunitz trypsin inhibitor OS Glycine max 24433.60 24408.10 91 12.5 2.00 0.30 4 0.06
O23957 Dehydrin OS Glycine max GN GmPM12 PE 2 S 17319.94 1858.29 13.5 3 1.41 0.28 2 0.06
C6T9Z5 Putative uncharacterized protein OS Glyc 43109.03 1650.38 28 11.5 1.41 0.27 2 0.06
Q84V19 Sucrose binding protein 2 OS Glycine max 56117.52 2226.42 65.75 23.25 1.93 0.25 4 0.05
O22121 Beta subunit of beta conglycinin Fragmen 47975.72 20300.60 200.5 32.75 2.00 0.25 4 0.05
P08170 Seed lipoxygenase 1 OS Glycine max GN LO 94597.61 3028.73 90.75 35.75 1.18 0.21 4 0.04
Q53B72 Putative chalcone isomerase 4 OS Glycine 23495.71 1460.83 11.5 2.5 0.01 0.20 2 0.04
Q43709 Bowman Birk proteinase isoinhibitor D II 12351.73 5458.41 27 4.5 2.00 0.20 4 0.04
Q9ZNZ4 Napin type 2S albumin 1 OS Glycine max P 18404.98 4001.44 39.75 10.5 2.00 0.18 4 0.04
Q94IA1 Kunitz trypsin inhibitor OS Glycine max 24333.52 34662.18 107 12 2.00 0.18 4 0.04
Q588Z3 Beta amylase OS Glycine max GN Gm BamyDa 56441.98 1367.07 58.5 22.75 2.00 0.16 4 0.03
C7EA91 Mutant glycinin subunit A1aB1b OS Glycin 44092.82 71745.98 326.25 33 0.42 0.15 4 0.03
Q70EL8 Dehydrin OS Glycine max GN lea D 11 PE 4 23733.70 9455.43 82.25 16.5 2.00 0.11 4 0.02
P93165 Em protein OS Glycine max PE 4 SV 1 11491.34 3266.78 22.25 6 0.06 0.11 4 0.02
C6TBB3 Putative uncharacterized protein OS Glyc 12345.51 1829.78 14.75 4.5 0.21 0.10 4 0.02
Q4LER6 Beta conglycinin alpha prime subunit OS 72513.18 26588.66 264.25 63.5 2.00 0.09 4 0.02
Q7M1N5 Glycinin A1aB1b Fragments OS Glycine max 6315.28 13404.83 14.5 3.5 1.09 0.07 4 0.01
P25272 Kunitz type trypsin inhibitor KTI1 OS Gl 22831.11 11727.02 70.75 12.5 0.16 0.06 4 0.01
C7EA92 Mutant glycinin subunit A1aB1b OS Glycin 43990.73 65306.21 275.25 30 0.70 0.05 4 0.01
C6T7Y4 Putative uncharacterized protein OS Glyc 33364.68 1386.46 32.5 13.5 2.00 0.04 4 0.01
Q5K3Q9 Putative dehydrin Fragment OS Glycine ma 20395.17 7625.21 70.5 15.75 2.00 0.00 4 0.00
P01071 Trypsin inhibitor B OS Glycine max PE 1 20268.75 17312.94 48.75 7.5 2.00 0.00 4 0.00
Q9FZP9 Alpha subunit of beta conglycinin Fragme 65199.76 25899.30 239.25 55.5   0.00 4 0.00
O22120 Alpha subunit of beta conglycinin Fragme 63221.91 105305.79 299.75 42.5   0.00 4 0.00
Q588Z5 Beta amylase OS Glycine max GN Gm BamyKz 56419.97 1193.51 46.25 20.75   0.00 4 0.00
Q588Z6 Beta amylase OS Glycine max GN Gm BamyTk 56499.03 945.94 42 20   0.00 4 0.00
Q588Z4 Beta amylase OS Glycine max GN Gm BamyTk 56426.96 1290.96 50.5 23.25   0.00 4 0.00
Q45UE7 Beta amylase OS Glycine max PE 2 SV 1 56412.98 1420.14 54.25 21.25   0.00 4 0.00
Q84UB3 Beta conglycinin alpha subunit Fragment 45018.62 24184.62 193.75 39.75   0.00 4 0.00
Q50JD8 Beta conglycinin beta subunit Fragment O 48387.22 22348.6 219 28.25   0.00 4 0.00
Q70EL7 Dehydrin OS Glycine max GN lea D 11 PE 3 25275.54 3943.08 30 8   0.00 3 0.00
Q70EL9 Dehydrin OS Glycine max GN lea D 11 PE 3 25551.97 3591.29 24 5.6666665   0.00 3 0.00
Q7M211 Glycinin A3B4 Plasmid pSPGD41 Fragment O 21495.15 29529.21 86 16.75   0.00 4 0.00
Q7M210 Glycinin A3B4 Plasmid pSPGL1 Fragment OS 27293.87 30284.32 105.25 23.75   0.00 4 0.00
Q6LBP7 Glycinin B 1b subunit 15 AA Fragment OS 1698.00 8521.25 7.6666665 2.3333333   0.00 3 0.00
P93707 Glycinin OS Glycine max GN Gly A3B4 PE 2 58633.58 45602.53 283 58.75   0.00 4 0.00
Q9LD16 Kunitz trypsin inhibitor 3 OS Glycine ma 24295.44 27398.43 66.25 9.25   0.00 4 0.00
Q39899 Kunitz trypsin inhibitor OS Glycine max 24308.45 17312.92 48.75 7.5   0.00 4 0.00
Q7XAW0 Lea protein OS Glycine max GN ZLDE 2 PE 25368.76 3593.48 33.75 8.5   0.00 4 0.00
Q39870 Lipoxygenase OS Glycine max GN lox2 PE 2 97551.04 754.83 39 25.5   0.00 2 0.00
C6T488 Putative uncharacterized protein OS Glyc 24403.62 35506 113.5 13.5   0.00 4 0.00
C6SX26 Putative uncharacterized protein OS Glyc 12914.35 3157.77 23.5 3.5   0.00 4 0.00
  1. The ScoreAVG is the average PLGS score for each hit. ProductsAVG is the average fragment ion products for a protein hit. PeptideAVG is the average of the peptide hits per identified protein. FmolCovariance is the covariance at the femtomole detection level for each protein in the replicate analyses. NgramAVG is the average (in nanograms) of each protein load on the column. The repetition rate is the repeatability of each protein in the replicates. % of TSP is the percentage of each protein in the total soluble mixture relative to the total protein load (in nanograms) on the column.