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Table 2 Bioreactor cultivations performed with the LAIV strain A/Singapore

From: Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells

 

CCI_4_1

CCI_4_2

CCI_6_1

CCI_6_2

CCI_8_1

CCI_8_2

CCI [viable cells/mL]

4.2 × 106

4.1 × 106

6.5 × 106

6.1 × 106

7.4 × 106

8.0 × 106

Trypsin conc. [units/cell]

10 × 10-6

1 × 10-6

1 × 10-6

1 × 10-6

10 × 10-6

10 × 10-6

Feed

-

-

-

-

-

200 mL

  1. CR.pIX cell cultures were infected at different CCI and trypsin activities. Upon infection, the temperature was reduced to 33°C for virus propagation.