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Table 2 Bioreactor cultivations performed with the LAIV strain A/Singapore

From: Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells

  CCI_4_1 CCI_4_2 CCI_6_1 CCI_6_2 CCI_8_1 CCI_8_2
CCI [viable cells/mL] 4.2 × 106 4.1 × 106 6.5 × 106 6.1 × 106 7.4 × 106 8.0 × 106
Trypsin conc. [units/cell] 10 × 10-6 1 × 10-6 1 × 10-6 1 × 10-6 10 × 10-6 10 × 10-6
Feed - - - - - 200 mL
  1. CR.pIX cell cultures were infected at different CCI and trypsin activities. Upon infection, the temperature was reduced to 33°C for virus propagation.