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Table 1 Temperature sensitivity of A/Singapore and B/Vienna viruses in CR.pIX cells

From: Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells

Maximum TCID50titers [virions/mL]* 33°C 37°C 40°C
Before adaptation to CR.pIX cells    
A/Singapore 1.3 × 108 0 -#
B/Vienna 1.8 × 105 0 -
After adaptation to CR.pIX cells    
A/Singapore 2.4 × 108 1.8 × 106 0
B/Vienna 3.2 × 106 0 -
  1. *Maximum titers of CR.pIX cells infected in T-flasks at a CCI of 2 × 106 cells/mL and with 1 × 10-6 units trypsin per cell.
  2. #not determined.