Phylogenetic analysis based on comparison of deduced amino acid sequence of EuIPI with those of other IPIs from different organisms including plants, bacteria, fungi, and animals (a) or with plant two type I IPIs (b). EuIPI shares a common evolutionary origin with other plant IPIs indicating that EuIPI belongs to the plant IPI group and has high homology with C. acuminata IPI2 (90.9% identity). Alignments were performed with Clustal W and visualized with TreeView. Distances between sequences are expressed as 0.1 changes per amino acid residue. Accession numbers and abbreviations: An, Aspergillus nidulans (AF479816); Ap, Acyrthosiphon pisum (FJ824667); At1, Arabidopsis thaliana (U47324); At2, Arabidopsis thaliana (U49259); Bc, Bupleurum chinense clone (GQ433719); Bm, Bombyx mori (AB274994), Ca1, Camptotheca acuminata (AF031079); Ca2, Camptotheca acuminata (AF031080); Ec, Escherichia coli (EU896065); Ce, Caenorhabditis elegans (AY597336); Eu, Eucommia ulmoides (AB041629); Hb1, Hevea Brasiliensis (AB294696); Hb2, Hevea Brasiliensis (AB294697); Hs, Homo sapiens (AF271720); Ik, Ipomoea sp. Kenyan (AB499048); Nc, Neurospora crassa (AB299023); Ng, Natronobacterium gregoryi (AJ564483); Nt1, Nicotiana tabacum (AB049815); Nt2, Nicotiana tabacum (AB049816); Ps, Periploca sepium (AB091677); Pt, Pinus taeda (GQ476784); Sl, Solanum lycopersicum (EU253957); Sp, Schizosaccharomyces pombe (U21154); and Zm, Zea mays (AF330034).