Figure 6

CLUSTAL multiple sequence alignment and secondary structure prediction for H1 A/California/07/2009, H1 A/New Caledonia/20/99, H1 A/Brisbane/59/2007 and H1 A/Solomon Islands/03/2006. A structure prediction was established using ExPASy Proteomics Tools (http://sequerome.georgetown.edu/sequerome, secondary structure prediction). The conserved cleavage site at R-324 is shown with an asterisk. The additional trypsin cleavage region is over-lined in red. The cleavage site K-419 identified from the digested fragments is indicated by a red arrow. Additional cleavage sites R-420, K-426 and K-427 are indicated by black arrows. The predicted coiled-coil region is indicated by the green over-line. The transmembrane domain is shown by the blue over-line. Amino acids that are divergent from the H1 rHA consensus sequence are highlighted in black. H1 A/California/07/2009 amino acid numbering is based on full length sequence.