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Figure 5 | BMC Biotechnology

Figure 5

From: Pandemic influenza vaccine: characterization of A/California/07/2009 (H1N1) recombinant hemagglutinin protein and insights into H1N1 antigen stability

Figure 5

Trypsin digestion of rHA proteins. rHA proteins were digested with 50 μg/mL trypsin for 30 minutes at 2-8°C. Approximately 1 μg of rHA protein was loaded per lane under reducing conditions. The two additional HA2 peptides (HA2a and HA2b) are indicated with arrows. Molecular weights of proteins are shown in kilodaltons. Panel A: Coomassie Blue-stained SDS-PAGE gel. Panel B: Western blot of trypsin-digested H1 A/California/07/2009 rHA protein. rHA protein was visualized using anti-H1N1 A/California/07/2009 serum from NIBSC (lot 09/152; sheep 506/507).

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BMC Biotechnology

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