Figure 1

Reducing and non-reducing SDS-PAGE of H1N1 rHA proteins. For each sample, the respective purified rHA protein was diluted to a concentration of 100 μg/mL in reducing or non-reducing SDS-PAGE sample buffer, and 1 μg was loaded per lane. The samples were separated using 4 – 12% gradient Nu-PAGE gels and stained with Coomassie Blue. HA0 represents full-length rHA protein and HA1 and HA2 peptide fragments of HA0. Molecular weights of proteins are shown in kilodaltons. rHA proteins were produced by Protein Sciences Corporation.