Table 2 Summary of major peptide fragments obtained from trypsin digestion of (A) nLac and (B) rLac with % Relative intensity, sequence and assigned glycan structures
A | |||
|---|---|---|---|
m/z | Relative Intensity | Peptide sequence | Assigned glycan structure |
1419.811 | 100 | GVTFDGGINSAILR | Â |
2126.243 | 87.55 | GRFPTGPTSPLSVINVTPGTK | |
1684.84 | 28.65 | SAGSSVYNYDNPVRR.D | Â |
3067.855 | 4.00 | VIEISIPGGTTGFPHPFHLHGHTFDVVR.S | |
2132.145 | 19.01 | DAVNTGGAGDNVTIR | (Hex) 1 (HexNAc) 1 (NeuGc) 1 |
B | |||
1419.531 | 96.35 | GVTFDGGINSAILR | Â |
1528.459 | 45.54 | SAGSSVYNYDNPVR | Â |
2125.837 | 24.34 | GRFPTGPTSPLSVINVTPGTK | Â |
2098.716 | 16.27 | YSFVLNANQPVDNYWIR | Â |
| Â | 12 | GPLVVYDPNDPHK | Â |
2593.952 | 1.61 | DAVNTGGAGDNVTIR | (Hex) 4 (HexNAc) 2 (Sulph) 1 |
| Â | Â | Â | (Hex) 4 (HexNAc) 2 (Phos) 1 |