Culture of AdCMV-EGFP and LeVSV-GCMV-EGFP transduced rat exocrine pancreas cells. (A-J) Culture of rat exocrine pancreas cells transduced with (A-E) AdCMV-EGFP MOI 1 and (F-J) LeVSV-GCMV-EGFP MOI 50, (A, F) day 2, (B, G) day 3, (C, H) day 4, (D, I) day 5 and (E, J) day 7 in culture. Adenoviral transduction resulted in higher transduction efficiencies and higher levels of EGFP expression on (A) day 2 and (B) day 3, when compared to the lentiviral vectors (F-G). However, from (C) day 4 on, massive cell death occurred in the adenovirally transduced condition and the monolayer formation was disturbed. On the contrary, the cell viability in lentivirally transduced cells remained normal and they had a strong and stable expression of the transgene.