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Figure 2 | BMC Biotechnology

Figure 2

From: Gene delivery to pancreatic exocrine cells in vivo and in vitro

Figure 2

Transduction of isolated rat exocrine pancreas cells using Mokola and VSV-G pseudotyped lentiviral vectors. (A-F) Day 3 in culture of transduced rat exocrine pancreas cells with LeMoCMV-EGFP (A) MOI 5, (B) MOI 10 or (D) LeVSV-GCMV-EGFP MOI 5, (E) MOI 10, (F) MOI 50. (C) Untransduced cells showed no fluorescence. The expression of the reporter increased with higher MOI and was better in LeVSV-GCMV-EGFP as compared to LeMoCMV-EGFP. Merged pictures of bright field and green fluorescence. (G) Quantification of the percentage of EGFP expressing cells on day 8 in culture after transduction with LeMoCMV-EGFP or LeVSV-GCMV-EGFP of rat exocrine pancreas on day 1.

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