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Table 4 Effects of components potentially responsible for the injurious effect of RPMI 1640

From: Aggravation of cold-induced injury in Vero-B4 cells by RPMI 1640 medium – Identification of the responsible medium components

 

LDH release (%)

 

Without deferoxamine

With deferoxamine

KHG

12 ± 08

02 ± 1

KHG + i-inositol

14 ± 09

01 ± 0

KHG + biotin

11 ± 06

02 ± 2

KHG + vitamin B12

12 ± 09

01 ± 1

KHG + p-aminobenzoic acid

13 ± 10

01 ± 0

  1. Components only present in RPMI 1640 or components of RPMI 1640 with concentrations far outside the range of the concentration in other media were added in identical concentration (inositol, 0.2 mM; biotin, 0.8×10-3 mM; vitamin B12, 4×10-6 mM; p-aminobenzoic acid, 7×10-3 mM) to Krebs-Henseleit buffer with 11.1 mM glucose (KHG). Cell injury was assessed by release of lactate dehydrogenase (LDH) at the end of cold incubation (168 h; n = 4).