Table 3 Composition of different cell culture media and Krebs-Henseleit buffer
RPMI | DMEM | L-15 | M199 | KHG | |
|---|---|---|---|---|---|
Na + | 139.0 | 157.0 | 145.1 | 144.1 | 143.6 |
K + | 5.3 | 5.8 | 5.8 | 5.4 | 5.9 |
Mg 2+ | 0.4 | 0.8 | 1.8 | 0.8 | 1.2 |
Ca 2+ | 0.4 | 1.8 | 1.3 | 1.8 | 2.5 |
Cl - | 109.5 | 120.9 | 146.8 | 125.8 | 128.3 |
SO 4 2- | 0.4 | 0.8 | 0.8 | 0.8 | 1.2 |
HCO3- | 24.0 | 44.0 | 14.3 | 26.2 | 25.0 |
H 2 PO 4 - /HPO 4 2- | 5.6 | 0.9 | 2.0 | 0.9 | 1.2 |
NO 3 - | 0.9 | 0.7*10 -3 | - | 4*10 -3 | - |
HEPES | - | - | - | - | 20.0 |
Glucose | 11.1 | 25.0 | 8.3 | 5.6 | 11.1 |
Galactose | - | - | 5.0 | - | - |
Deoxyribose | - | - | - | 4*10-3 | - |
Ribose | - | - | - | 3*10-3 | - |
p-Aminobenzoic Acid | 7*10 -3 | - | - | 0.4*10 -3 | - |
Biotin | 0.8*10 -3 | - | - | 4*10 -5 | - |
Choline | 20*10-3 | 29*10-3 | 10*10-3 | 4*10-3 | - |
Folic Acid | 2*10-3 | 9*10-3 | 2*10-3 | 2*10-5 | - |
i-Inositol | 0.2 | 40*10 -3 | 10*10 -3 | 0.3*10 -3 | - |
Niacin | - | - | - | 0.2*10-3 | - |
Niacinamide | 8*10-3 | 33*10-3 | 10.0*10-3 | 0.2*10-3 | - |
Pantothenate | 0.5*10-3 | 8*10-3 | 4*10-3 | 2*10-5 | - |
Pyridoxine | 5*10-3 | 20*10-3 | 5*10-3 | 0.1*10 -3 | - |
Riboflavin | 5*10-4 | 1*10-3 | - | 3*10-5 | - |
Thiamine | 3*10-3 | 12*10-3 | 3*10-3 | 3*10-5 | - |
Vitamin B 12 | 4*10 -6 | - | - | - | - |
Glutathione | 3*10 -3 | - | - | 2*10 -3 | - |
Pyruvate | - | 1.0 | 5.0 | - | - |
Phenol red | 13*10-3 | 40*10-3 | 30*10-3 | 50*10-3 | - |
Vitamin A | - | - | - | 3*10-5 | - |
Calciferol | - | - | - | 0.3*10-3 | - |
Menadione | - | - | - | 6*10-5 | - |
α-Tocopherol phosphate | - | - | - | 2*10-5 | - |
Ascorbic acid | - | - | - | 0.3*10-3 | - |
ATP | - | - | - | 2*10-3 | - |
AMP | - | - | - | 0.6*10-3 | - |
Adenine sulfate | - | - | - | 30*10-3 | - |
Guanine | - | - | - | 2*10-3 | - |
Hypoxanthine | - | - | - | 2*10-3 | - |
Thymine | - | - | - | 2*10-3 | - |
Uracil | - | - | - | 3*10-3 | - |
Xanthine | - | - | - | 2*10-3 | - |
Pyridoxal | - | - | - | 1*10-4 | - |
Cholesterol | - | - | - | 5*10-4 | - |
L-Arginine | 1.2 | 0.4 | 2.5 | 0.3 | - |
L-Asparagine | 0.4 | - | 1.9 | - | - |
L-Aspartic acid | 0.2 | - | - | 0. 5 | - |
L-Cysteine | - | - | 1 | 1*10-3 | - |
Cystine | 0.2 | 0.2 | - | 0.1 | - |
L-Glutamic acid | 0.1 | - | - | 0.9 | - |
L-Glutamine | 2.1 | 4.0 | 2.1 | 0.7 | - |
Glycine | 0.1 | 0.4 | 2.7 | 0.7 | - |
L-Histidine | 0.1 | 0.2 | 1.6 | 0.1 | - |
L-Hydroxyproline | 0.2 | - | - | 0.1 | - |
L-Isoleucine | 0.4 | 0.8 | 1.0 | 0.3 | - |
L-Leucine | 0.4 | 0.8 | 1.0 | 0.9 | - |
L-Lysine | 0.3 | 0.8 | 0.6 | 0.4 | - |
L-Methionine | 0.1 | 0.2 | 0.5 | 0.2 | - |
L-Phenylalanine | 0.1 | 0.4 | 0.8 | 0.3 | - |
L-Proline | 0.2 | - | - | 0.4 | - |
L-Serine | 0.3 | 0.4 | 1.9 | 0.5 | - |
L-Threonine | 0.2 | 0.8 | 2.5 | 0.5 | - |
L-Tryptophan | 25*10-3 | 0.1 | 0.1 | 0.1 | - |
L-Tyrosine | 0.1 | 0.4 | 1.7 | 0.2 | - |
L-Valine | 0.2 | 0.8 | 0.9 | 0.4 | - |