| LDH release (%) |
---|
Solution | Without deferoxamine | With deferoxamine |
---|
RPMI | 66 ± 26 | 01 ± 1 |
RPMI without supplements | 76 ± 23 | 02 ± 1 |
- Vero-B4 cells were incubated at 4°C in RPMI 1640 supplemented with fetal bovine serum (10%) and penicillin/streptomycin (25 U ml-1/25 μg ml-1) or in “pure” RPMI 1640 without supplements for 168 h. The iron chelator deferoxamine (1 mM) was added to part of the incubations. Lactate dehydrogenase (LDH) release was assessed after cold storage (n=4).