Aggravation of cold-induced injury in Vero-B4 cells by RPMI 1640 medium – Identification of the responsible medium components

Table 1 Comparison of RPMI 1640 from two different companies

	LDH release (%)
Conditions of cold incubation	RPMI (Gibco)	RPMI (Sigma)
No Inhibitor	92 ± 6	89 ± 4
+ Deferoxamine	01 ± 1	02 ± 0
+ Trifluoperazine + fructose	01 ± 1	01 ± 1
+ Ethanol (solvent control)	90 ± 1	88 ± 4

Vero-B4 cells were incubated in RPMI 1640 purchased from Gibco or Sigma at 4°C for 168 h. The iron chelator deferoxamine (1 mM) or trifluoperazine (20 μM) plus fructose (10 mM), the latter as inhibitor combination of mitochondrial permeability transition [30], were added to part of the incubations during cold storage. Ethanol was used as solvent control for trifluoperazine. Cell injury was assessed by release of lactate dehydrogenase (LDH) after cold incubation (n = 4).

ISSN: 1472-6750