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Figure 3 | BMC Biotechnology

Figure 3

From: Aggravation of cold-induced injury in Vero-B4 cells by RPMI 1640 medium – Identification of the responsible medium components

Figure 3

Influence of modified Krebs-Henseleit buffer on cold-induced injury to Vero-B4 cells. Vero-B4 cells were stored at 4°C for 168 hours in RPMI 1640, Krebs-Henseleit buffer (KH), modified KH buffer with either 11.1 mM glucose (KHG), low Ca2+ concentration (KH(Ca-); 0.42 mM) or high inorganic phosphate concentration (KH(P+); 5.6 mM) alone or in different combinations (KHG(Ca-,P+), KHG(Ca-), KHG(P+), KH(Ca-,P+)). Some cells were stored in the presence of the iron chelator deferoxamine (1 mM, striped bars). Cell injury was assessed by the release of lactate dehydrogenase (LDH; n = 4) directly after cold storage (168 h).

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