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Figure 4 | BMC Biotechnology

Figure 4

From: A one-step cloning method for the construction of somatic cell gene targeting vectors: application to production of human knockout cell lines

Figure 4

KO cell lines were confirmed by western blot . (a) Western blots of wild-type, heterozygous and homozygous SirT1 KO cell lines from HCT116 cells using anti-SirT1 and anti-β-actin antibodies. WT indicates parental cells, WT/KO indicates SirT1 heterozygous KO cells, KO/KO indicates SirT1 homozygous KO cells. (b) Western blots of wild-type and homozygous HDAC2 KO cell lines from DLD1 cells using anti-HDAC2 and anti-β-actin antibodies. WT indicates parental cells, WT/KO indicates HDAC2 heterozygous KO cells, KO/KO indicates HDAC2 homozygous KO cells.

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